Autofocus video microscopes are efficient tools for industrial inspection, scientific research, and quality control. With their automated focusing capabilities and real-time video imaging, they significantly improve observation efficiency. However, not all scenarios are suitable for the use of such equipment.
Autofocus Video Microscope Optical Features
1. Fast autofocus: No manual focusing required, significantly improving detection efficiency.
2. Real-time video observation: Supports dynamic process observation and recording.
3. Limited depth of field: At high magnifications, the depth of field is shallow, requiring high sample flatness.
4. Dependent on surface texture/features: The autofocus algorithm relies on changes in brightness or structural features on the sample surface.
5. Sensitive to Vibration: Even minor vibrations may cause focusing instability or image blurring.
6. Adjustable Lighting: Four-zone adjustable lighting ensures uniform illumination, with automatic exposure reducing reflections and producing a soft effect.
Typical Application Scenarios
1. Batch product appearance inspection: electronic components (PCB solder joints, chip pins), precision parts (threads, small holes), and scratch and stain detection on jewelry.
2. Surface morphology analysis: Measurement of material surface roughness and scratch depth (requires 3D analysis software).
3. Dynamic process recording: Observation of micro-mechanical motion and material deformation under heat.
4. Educational demonstrations: Real-time display of microscopic structures for simultaneous observation by multiple users.
5. Laboratory routine testing: Rapid screening of biological samples and material microstructures.
Not Applicable Scenarios
1. Completely transparent/low-contrast samples: Glass, transparent plastic, unstained cells, etc., lack surface features, making it difficult for the autofocus system to find sufficient contrast for focusing.
2. Highly reflective or mirror-like surfaces: Highly reflective metals, mirrors, etc., cause strong light reflection, interfering with imaging and causing the autofocus system to malfunction or misjudge.
3. Deep holes or complex three-dimensional structures: Insufficient light in deep holes and obstructed views by internal wall structures make it difficult for the autofocus system to locate the effective focal plane.
4. High-speed movement or instantaneous changes: The autofocus system requires time to calculate and adjust, making it difficult to keep up with rapid changes, resulting in blurry images or missed critical frames.
5. High-temperature or extreme environment samples: Thermal radiation interferes with imaging, and high temperatures may damage the lens or sensor (most devices have an upper temperature limit of approximately 40°C–50°C).
6. Scenes requiring extremely large depth of field: Under high magnification, the depth of field is only micrometer-level, making it impossible to clearly display areas with significant height differences simultaneously.
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